Phytochemical Screening and Acetylcholinesterase and Butyrylcholinesterase Inhibitory and Thrombolytic Activities of Grewia abutilifolia Vent. & Juss. Leaf

Md. Rajdoula Rafe, Rayhanus Salam, Md. Monir Hossain, Mohammad Mehedi Masud

Abstract


Cholinesterase inhibitors offer the possible pathway to fight against various types of
neurodegenerative diseases. Grewia abutilifolia is used extensively in traditional and folk medicines mainly to
improve intellectual capabilities and memory enhancement purposes which support its possible effects against
neurodegenerative disorders. We performed preliminary phytochemical screening to observe the presence of potential
phytoconstituents of G. abutilifolia leaf extracts and evaluated acetylcholinesterase and butyrylcholinesterase
inhibitory properties to measure its anti-cholinesterase activities. Preliminary phytochemical screening confirmed the
presence of saponins, tannins, flavonoids, terpenoids, steroids, carbohydrates, coumarins, alkaloids etc. The
petroleum ether (PESF), chloroform (CSF) and aqueous soluble fractions (AQSF) of the crude extract of the plant
leaf were screened for bioactivities. The highest inhibition of acetylcholinesterase was exhibited by aqueous soluble
fraction (AQSF) with IC50 = 5.73 ± 0.59 μg/ml, while the highest inhibition of butyrylcholinesterase was shown by
petroleum ether soluble fraction (PESF) with IC50 = 6.57 ± 1.81 μg/ml in a dose-dependent manner. During assay for
thrombolytic activity, the chloroform soluble fraction (CSF) revealed highest activity with 25.75 ± 1.62% clot lysis.
These results of the present study suggested the effectiveness of G. abutilifolia against various neurological diseases.


Keywords


Phytochemical screening, cholinesterase inhibition, thrombolytic, Grewia abutilifolia.

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The Dhaka University Journal of Pharmaceutical Sciences
ISSN 1816-1820 (Print) 1816-1839 (Online)