Neuroprotective Properties of Nigella sativa (L.) Seeds Extract in Sprague Dawley Rats models

Zahir Uddin Babar, Wan Mohd Azizi Wan Sulaiman, Jalal Uddin, Zubair Khalid Labu

Abstract


Neuroinflammation has been closely linked to neurodegeneration that leads to dysfunction of memory
and learning where glial fibrillary acidic protein (GFAP) plays important role positively within hippocampus. In
experimental neurodegeneration achieved by two-vessel occlusion (2VO) intervention, Nigella sativa (L.) seeds
extract (50 mg/kg/day orally) has shown to have anti-neuroinflammatory and antioxidant properties. The brain
hippocampal tissues were humanely collected at the end of 10th treatment week and preserved in Allprotect™
reagent at –80°C. Total RNA was extracted and purified by phenol/chloroform method using kits and reverse
transcribed into cDNA and relatively quantified as per ΔΔCq. The NSSE treatment showed significantly (p<0.001)
different relative GFAP mRNA expression in the treated group as compared to that of untreated 2VO whilst it was
insignificantly (p>0.5) different to that of healthy control (HC). Prolonged or daily treatment with NSSE (Nigella
sativa (L.) seeds extract) may provide moderate anti-neuroprotective activity within hippocampus.


Keywords


Nigella sativa, neuroinflammation, two-vessel occlusion, glial fibrillary acidic protein, hippocampus, reverse transcription quantitative PCR (RT-qPCR).

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The Dhaka University Journal of Pharmaceutical Sciences
ISSN 1816-1820 (Print) 1816-1839 (Online)