Determination of percent incorporation of radio tracer (14C-Acetate) in newly synthesized macromolecules in different tissues of streptozotocin induced diabetic rat vis-control rats

Abstract

The effect of streptozotocin induced diabetes on the metabolic pattern o f ‘ ‘’C-acetate
was investigated by IP injection of uniformly labelled >‘*C-acetate and compared with
sham controls. Significant metabolic changes were observed, synthesis of protein in
SZID heart, liver, muscle and kidney was decreased by 99%, 97% and 99% and 98%
respectively Synthesis of phospholipid, glycolipid and cholesterol of SZID brain was
decreased by 67%, 62% and 90% respectively. Synthesis of glycogen in SZID liver and
muscle was decreased by 34% and 99% while the incorporation of radio activity in
newly synthesized lipid in adipose tissue, muscle and liver was depressed by 99%,
99% and 88% respectively.