Silver nanoparticles based fluorimetric estimation of carbamazepine in pharmaceutical formulations
Keywords:
Carbamazepine, Silver nanoparticles, Fluore-scence, Quenching, Pharmaceuticals.Abstract
A sensitive and selective fluorimetric estimation of carbamazepine (CBZ) using silver nanoparticles as emission reagent has been investigated. In this study, silver nanoparticle has been prepared based on aqueous-gaseous phase reaction of silver nitrate solution and ammonia gas. The nanoparticles are water soluble, stable and have extremely narrow emission band. These are used as fluorescence probe for the assay of carbamazepine based on the quenching effect of carbamazepine on the emission of silver nanoparticles. The principal reason for quenching is likely to be a complexation between carbamazepine and silver nanoparticles. Under the optimum conditions, the quenched fluorescence intensity is linear with the concentration of carbamazepine in the range of 3×10-6 M to 1.0×10-4 M (r = −0.9987) with a detection limit of 1.5×10-7 M. The proposed method has been applied to the determination of carbamazepine in commercial pharmaceutical formulations.
