Antibacterial and cytotoxic constituents from bridelia verrucosa haines growing in Bangladesh

Abstract

Fractionation and purification of methanolic extract of stem bark of the Bridelia verrucosa growing
in Bangladesh afforded glochidonol (1), brassicasterol (2), friedelin (3), 5β-24S-ethylchoestane-3β-ol (4),
stigmasterol (5), ketooleanane (6), 5-5'-dihydroxy sesamine (7) and pinoresinol (8). Compounds 1, 2, 3, 4, 6 and 8
are the first report of their isolation from the plant while compound 7 appear to be a new isolate from natural
source. The isolated compounds exhibited cytotoxic activity against brine shrimp nauplii having significant LC50
and LC90 values and moderate to strong antimicrobial activity against 13 Gram positive and Gram negative
bacterial strains and 3 fungi. Compound 8 showed highest inhibition of growth of microorganisms with zone of
inhibition of 16.7 mm against Shigella dysenteriae. Compounds 1-4 and 6-8 also revealed moderate free radical
scavenging activity, in DPPH assay.