Standardization of adhatoda vasica nees market preparations by RP-HPLC method

Abstract

In recent times, quality control of herbal and traditional medicines with modern scientific techniques
and knowledge are of great concern. The present study reveals a simple and improved reversed phase HPLC method
for qualitative analyses of Adhatoda vasica Nees market preparations via quantitation of its major metabolite,
vasicine as a marker compound. Three market preparations, each of four different herbal and traditional
manufacturers, were analyzed. The market preparations were extracted with chloroform and the residue obtained
from extraction of each market preparation was analyzed for quantitation of vasicine by RP-HPLC method with ODS
column using a mixture of water and methanol (60:40) as mobile phase at a flow rate of 0.5 ml/min. The estimated
quantities of vasicine compared to reference standard for marketed products of four different manufacturers were
found to be 1.502 ± 0.064 g, 1.590 ± 0.081 g, 1.761 ± 0.061 g and 1.627 ± 0.082 g, respectively per 100 ml of
preparation.