In vivo studies of protein binding of ketorolac in rat model by UV-VIS spectrophotometry and high performance liquid chromatographic methods

Abstract

In this study, an attempt has been made to determine the protein binding of ketorolac at different time and
concentration in rat model. A total of ten rats were used for this study. The rats were subdivided into two groups and 100μg/ml
and 200μg/ml of ketorolac were administered through the intra-peritoneal route to rats of group 1 and group 2, respectively. The
serum from the rats were collected and analyzed using UV-VIS spectrophotometry and HPLC. In the UV-VIS
Spectrophotometric study, the highest percentage of protein binding for ketorolac was found to be 63.067% in group 1 and
74.63% in group 2. In case of HPLC method, the highest protein binding of ketorolac for group 1 and group 2 were 89.72% and
95.07%, respectively. The observed protein binding percentage of ketorolac by HPLC method was slightly higher than that
found in UV-VIS Spectrophotometric method. The result obtained in this study showed that the protein binding of ketorolac
changes with the time and drug concentration. The percentage of protein binding was higher for higher drug concentration.