Synthesis and biological evaluation of 2-methyl 1hbenzimidazole and 1h-benzimidazol-2-yl-methanol
Keywords:
Benzimidazole, NMR spectroscopy, Antioxidant activity, Brine shrimp lethality, Antimicrobial.Abstract
Two benzimidazole derivatives namely 2-methyl-1H-benzimidazole (1) and 1H-benzimidazol-2-
ylmethanol (2) were synthesized in high yields by condensing O-phenylenediamine with two different carboxylic
acid derivatives: glacial acetic acid and glycolic acid, following a different synthetic protocol. Structures were
elucidated by spectroscopic methods. Among the two benzimidazole derivatives synthesized, 2-methyl-1Hbenzimidazole
(1) showed moderate antioxidant activity with IC50 values of 144.84μg/ml whereas 1H-benzimidazol-
2-ylmethanol (2) displayed mild antioxidant activity (IC50 value 400.42 μg/ml). Compound 1 also exhibited
prominent cytotoxic activities with the LC50 value of 0.42 μg/ml when compared to the standard vincristine sulphate
(LC50 value of 0.544 μg/ ml). It also demonstrated weak antimicrobial activity having 7-8 mm of zone of inhibition.
