In vitro toxicity study of reconstituted amphotericin b - lipid derivatives dry powder for nebulization
Keywords:
Amphotericin B, Cytotoxicity, Lipid drug carriers, Alveolar macrophage, Nebulizer.Abstract
The aim of the present research work was to evaluate the safety of reconstituted dry powder
amphotericin B (AmB) inhalation via nebulizer. This study was carried out on respiratory cell lines (A549, Calu-3,
NR 8383), kidney cells (293T/17), human red blood cells (RBC) and aerosol properties were determined by
Andersen Cascade Impactor (ACI). AmB, a lipid derivative reconstituted dry powder was formulated by
lyophilization and reconstituted into distilled water at AmB concentration at 4 mg/ml. The value of MMAD, FPF
were obtained as 1.7 to 2.05 μm and 70 to 80%, respectively. The cytotoxicity test carried out by MTT assay of
lipid formulations revealed a very low toxicity on respiratory cell lines such as kidney cells, than pure AmB at
concentration 1 to 8 μg/ml of AmB. In-vitro cytotoxicity results showed less toxicity to human red blood cells
(RBC) than pure AmB at concentration 1 to 8 μg/ml of AmB.
