Development and Optimization of Acyclovir Loaded Mucoadhesive Microspheres by Box – Behnken Design
Keywords:
Acyclovir, Box-Behnken Design, kinetic models, microspheres, mucoadhesion, optimization.Abstract
The grail of the study was to design, develop and characterize sustained release mucoadhesive
microspheres of acyclovir and to optimize the drug release profile using response surface methodology by applying
Box–Behnken design (BBD) which was equipped with three levels and three factors. Microspheres were prepared
from Methocel K15M and Ethocel Standard 45 Premium using the emulsification solvent evaporation technique. The
independent factors were the amounts of Methocel K15M (X1), amount of Ethocel Standard 45 Premium (X2), and
RPM (X3). The dependent variables were cumulative percentage drug release (CDR) at 8 hour (Y1), bond strength
(Y2), and swelling at 4 hour (Y3). To understand the effects of different factor level combinations on the responses,
various response surface graphs and contour plots were prepared. Predicted values and experimental values for
optimized formulation (X1 = 600 mg, X2 = 500 mg, and X3 = 336.57) was found to be in close agreement.
