Secondary Metabolites and Antioxidant Potentials of Axis Sea Bamboo (Isis hippuris)

Abstract

Secondary metabolites of the axis section (modulla skeleton) of sea bamboo (Isis hippuris) were
identified by GC-MS. The dominant compounds using GC-MS in the ethanol fraction were hexanedioic acid
(11.85%), acetamide (9.46%), n-hexadecanoic acid (9.22%) and thiosulfuric acid (7.22%). On the other side, the
dominant compounds of the test results in the ethyl acetate fraction were 7-oxabicyclo[4.1.0] heptanes (28.27%), 1,2-
benzene dicarboxylic acid (14.77%), cis-8-(N-pyrrolidyl)-(2,2,5,5-tetradeutero)bicyclo[4.3.0]nona-3,7-diene (9.98%),
hexahydropyridine (7,86%). The dominant compounds of the n-hexane fraction were hexanedioic acid (41.99%),
azetidine with a peak number of 40 (9.98%), 1-octadecene (8,36%). Antioxidant activities were also evaluated by
DPPH scavenging assay. The ethanol, n-hexane and ethyl acetate fractions showed total flavonoid contents of 7.86%
± 0.12, 12.97% ± 0.36, 1.88% ± 0.26, respectively and the IC50 in the antioxidant assay were 480.25±74,74,
469.50±19,13, 3221.07±138,69 respectively.