Cholinesterase and Glycation Inhibition Assay of Several Metabolites Obtained from Plant and Fungi

Abstract

Cholinesterase inhibition is one of the major strategies to develop better quality anti-Alzheimer’s drug,
while inhibition of glycation pathway is a useful therapeutic strategy to treat diabetic complications. This study was
designed to evaluate the anti-acetylcholinesterase, anti-butyrylcholinesterase and anti-glycation activities of 8
secondary metabolites namely, RS-8, RS-22C (arborinine), CL-1 (betulinic acid), CL-2 (ursolic acid), CCL-1
(cajaninstilbene acid), CP-1 (beta-sitosterol), HS-1 (anhydrofusarubin) and HS-2 (methylether of fusarubin). It was
found that RS-8 and arborinine showed promising activity in inhibiting acetylcholinesterase with IC50 values of 24.40
 0.39 and 13.14  0.07, respectively. Anhydrofusarubin and methyl ether of fusarubin exhibited moderate activities
with IC50 values of 47.82  0.54 and 44.58  0.8. Arborinine and cajaninstilbene acid potentially inhibited
butyrylcholinesterase with IC50 values of 26.34  0.31 and 25.82  0.34, respectively, but other metabolites did not
show such inhibitory activities. Inhibition of glycation process was evaluated by bovine serum albumin assay but
none of the metabolites significantly inhibited the glycation pathway.