Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Hydrochlorothiazide and Losartan Potassium in Tablet Dosage Form

Abstract

In the present study, a simple, sensitive and specific liquid chromatography (RP-HPLC) method has
been developed and validated for the quantification of hydrochlorothiazide and losartan potassium in tablet dosage
form. A shim-pack CLC-ODS column (250 mm X 4.6 mm, 5μ and a mobile phase constituting 0.025 M phosphoric
acid solution: acetonitrile (60:40 v/v, pH 3.0 adjusted with 80% phosphoric acid) were used. The flow rate was 1.5
ml/min and detection was carried by using ultraviolet (UV) detector at a wavelength of 254 nm. The retention times
of hydrochlorothiazide and losartan potassium were 3.748 min and 8.790 min, respectively. The peaks of
hydrochlorothiazide and losartan potassium were well separated (resolution 22.17). The calibration curves were linear
over the concentration range of 80% to 120% (R2 > 0.999 for both the drugs). The proposed method is accurate with
100.165% recovery for hydrochlorothiazide and 100.422% recovery for losartan potassium and precise (% RSD <
0.5). The proposed method was successfully applied for the estimation of hydrochlorothiazide and losartan potassium
in market products (three brands) and potency was found within limit. Therefore, this method can be a convenient
and efficient option for the analysis of hydrochlorothiazide and losartan potassium in tablet dosage form.