Development and Validation of Bioanalytical HPLC Method For Estimation of Telmisartan In Rat Plasma: Application To Pharmacokinetic Studies

Abstract

A simple, specific, sensitive and rapid reversed phase high performance liquid chromatographic
(HPLC) method has been developed and validated for the determination of telmisartan in small volumes of rat
plasma. Biological sample preparation involving simple extraction with organic solvent, followed by dilution with
mobile phase was adopted to eliminate any chromatographic solvent effects. The method was proven to be linear over
a plasma concentration range of 10 to 1000 ng/mL with a mean correlation coefficient of 0.9942. The limit of
detection and the limit of quantification of the newly developed method were determined to be 1 ng/mL and 10
ng/mL, respectively. The method was successfully applied to assess pharmacokinetic parameters of telmisartan in
Wister rats following a single oral dose (1.8 mg/kg, b.w.). The developed method was established as a rapid
analytical tool in a pharmacokinetic study as it required short retention time, high precision, sensitivity and small
volumes of plasma for analysis.