First Derivative Synchronous Spectrofluorimetric Quantification of Telmisartan/Amlodipine Besylate Combination in Tablets
Keywords:
Amlodipine besylate, Telmisartan, First derivative, Synchronous, Spectrofluorimetry.Abstract
A first derivative synchronous spectrofluorimetric method has been developed and validated for
simultaneous determination of telmisartan (TEL) and amlodipine besylate (AML) in combined tablet dosage form
without any prior separation of components from the sample. TEL was determined at emission wavelength of 675
nm (zero-crossing wavelength point of AML). Similarly, AML was measured at 458 nm (zero-crossing wavelength
point of TEL). The first derivative amplitude- concentration plots were rectilinear over the range of 4-14 μg/ml for
TEL and 1-6 μg/ml for AML. The method was validated statistically as per ICH guidelines. Limit of detection
(LOD) and quantification (LOQ) are reported. The % assay in commercial formulation was found to be in the range
99.60 – 100.22 and 98.40 – 99.80 for TEL and AML, respectively by the proposed method and % RSD values for
precision and accuracy studies were found to be less than 2. The proposed method can be successfully applied for
routine analysis of TEL and AML in tablets.
