Studies on Chemical Constituents and Biological Activities of an Endophytic Fungi from Magnifera indica L.
Keywords:
Endophytic fungi, ergosterol, antimicrobial activity, general toxicity, antioxidant activity.Abstract
Two compounds, ergosterol (1) and 4-hydroxy-hexadec-6-enoic acid methyl ester (2) were isolated
from the ethyl acetate extract of the endophytic fungal strain labeled as MI-3, isolated from the leave of Magnifera
indica L. The structures of the isolated compounds were elucidated by 1H NMR studies and comparing with
published data. The crude ethyl acetate extract, three column fractions and ergosterol were tested for antimicrobial
activity against five Gram-positive and eight Gram-negative bacteria and three fungi by disc diffusion method. The
general toxicity and antioxidant activity of the parent extract, column fractions and ergosterol were also evaluated by
using brine shrimp lethality assay and free radical scavenging assay, respectively. Low activities were observed in all
cases.
