Isolation and Charaterization of Bioemulgent from the Fruit Pulp of Prunus insititia

Abstract

The aim of the research work was to isolate biopolymer and to evaluate its emulsifying ability by
formulating o/w type of emulsion. The biopolymer was isolated from the fruit pulp of Prunus insititia by treating
with water. The aqueous extract was treated with an optimized ratio of dimethyl ketone and kept in refrigeration for 6
hours. The biopolymer was collected by centrifugation, dried and subjected to physicochemical analysis like
solubility, pH, colour, viscosity. Eight different emulsions of o/w type were prepared by using liquid paraffin oil and
biopolymer in various concentrations ranging from 50 mg to 1 gm. The formulated emulsions were subjected for
various evaluation parameters like globule size, pH, the effect of freezing and thawing cycle, effect of centrifugation,
etc. The results were compared with the standard emulsion which was prepared by using acacia as an emulsifier. The
emulsions were not showing any significant stability due to increase in the globule size. The final conclusion can be
drawn that the biopolymer as an emulsifier in lower concentration (50 and 100 mg) showed its potential emulsifying
property in the formulation FE1 and FE2 with uniform globule size ranging from 12 to 30 μm and stable for 3
freezing and thawing cycles.