Culture, reproduction and embryogenesis of wild zebrafish (danio rerio) in laboratory condition

Abstract

Zebrafish (Danio rerio: Hamilton, 1822) is a well‐known model organism of
biological research. Generally it is found in the fresh water ponds. However, the
culture, breeding and embryonic development of wild D. rerio of Bangladesh is
scarcely represented in the literature. Therefore, a laboratory experiment was
conducted to study these aspects of local zebrafish. Water quality parameters for
adult fish husbandry system were recorded. These were temperature (°C) 29.18 ±
1.53, pH 7.55 ± 0.144, TDS (ppm) 225 ± 11.136, (ORP) 48.93 ± 26.04, DO (ppm) 1.24 ±
0.591, conductivity (mS/cm) 0.450 ± 0.022, are resistivity (Mῼcm) 0.0026 ± 0.00049
for adult fish tanks. The parameters obtained from larvae rearing ranks were
temperature (°C) 29.56 ± 0.777, pH 7.73 ± 0.421, TDS (ppm) 198.33 ± 39.627, (ORP)
51.37 ± 17.387, DO (ppm) 2.02 ± 0.694, conductivity (mS/cm) 0.397 ± 0.079,
resistivity (MΩcm) 0.0026 ± 0.00044. After one month of rearing, adult male and
female fishes were selected for natural breeding and spawning and obtained 87.5 ±
7.72% spawning success, 96.83±0.81% fertilization rate and 94.89 ± 0.27% hatching
rate. The embryonic development for zygote, cleavage, blastula, gastrula,
segmentation and pharyngula periods were observed and obtained proper
development for each state at 28.5ºC. The survival rate of hatchlings was 10 ‐ 20%
up to 20 days post fertilization. The higher spawning success, fertilization rate,
hatching rate and survival rate indicate the possibility and potentiality of culturing
zebrafish in laboratory conditions and could be established as a model animal for
Bangladeshi researchers.