16S rRNA gene sequence based identification of vibrio spp. in shrimp and tilapia hatcheries of Bangladesh

Abstract

Presence of Vibrio spp., one of the deadliest fish and shrimp pathogens in
aquaculture facilities worldwide for which hatchery owners often suffer hectic
economic losses, were identified in shrimp and tilapia hatcheries of Cox’s Bazar
and Mymensingh, Bangladesh. Thirty seven Vibrio isolates, selected on the basis
of their morphological dissimilarities in thiosulphate citrate bile salt sucrose
agar (TCBS) plate, were subjected to amplified 16S ribosomal DNA restriction
analysis (ARDRA) by using AluI restriction enzyme following their DNA
extraction and amplification of 16S rRNA (1450 bp). From this analysis,
representative isolates of 8 ARDRA groups, named as ARH 1 to ARH 8, of
different band patterns were sequenced and identified as Vibrio alginolyticus,
Aeromonas veronii, A. hydrophila, Vibrio vulnificus, V. cholerae, Edwardsiella
hoshinae, Bacillus methylotrophicus and Aeromonas veronii, respectively. Vibrio
species identified in this study are pathogenic for human and aquatic
organisms, and were found only in shrimp hatchery with the dominance of V.
alginolyticus. Findings of this study indicate the poor quality of water treatment
and management of the hatchery. It was also observed that all the three Vibrio
species were present in the Artemia rearing tank which indicates the possible
source of pathogens.