Isolation of Quercetin-3-O-beta-d-glucopyranoside from the Leaves of Azadirachta Indica and Antimicrobial and Cytotoxic screening of the Crude Extracts

Abstract

Quercetin-3-O-beta-D-glucopyranoside was isolated from the ethylacetate soluble fraction of the ethanol extract of the fresh leaves of Azadirachta indica (Family: Meliaceae). The crude extract of hexane, ethylacetate and butanol soluble fractions of this ethanol extract were subjected to antimicrobial screening and brine shrimp lethality bioassay. The ethylacetate crude extract exhibited moderate antimicrobial activity against most of the test organisms and also showed significant cytotoxicity having LC₅₀ 0.61 µg/ml.