Residual DDTs and Fatty Acid Compositions in Popular Two Sea Fish Samples

M. I. R. Mamun, Nilufar Nahar


Fatty acid compositions, DDT and its metabolites of popular two sea fishes i.e., Pampus argenteus (Pomfret) and Lates calcarifer
(Vernacular) were determined. Lipid was extracted by ethylacetate, saponified and converted to methyl ester using BF3-MeOH to determine
fatty acid compositions by GC-FID.For DDTs,edible parts of the fish samples were extracted by solid phase dispersion method, cleaned up
with concentrated H2SO4 and finally analyzed by GC-ECD. The LOD and LOQ for DDE, DDD, 2, 4´-DDT and 4, 4´-DDT were found to be
0.39, 0.39, 1.56, 1.56 and 1.36, 1.36, 4.89 and 4.89 μg/kg respectively. Internal Standard CB53 (0.25mg/g) was used to determine the
recovery of the experiment and was within range of 77.48-92.35 %. The residual amount of DDT (2, 4´-DDT+4, 4´-DDT), DDE and DDD
in pomfret and vernacular were found to be in the range of 13.56-81.05, 3.86-21.08, 4.32-35.4, and 23.44-85.59, 2.90-5.81 and 6.33-12.44
μg/kg respectively. The ratios of DDT/ΣDDTs in pomfret and vernacular were 0.675 and 0.773 respectively. Among the saturated fatty
acids, palmitic acid was predominant (62.84% for pomfret and 41.81% for vernacular) whereas among the unsaturated fatty acids oleic acid
was predominate and higher in pomfret (15.11 %).


DDTs, Fatty acid, Pomfret, Vernacular and GC.

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Dhaka University Journal of Science ISSN 1022-2502 (Print) 2408-8528 (Online)